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Published November 1947 | Published
Journal Article Open

The colorimetric determination of hexoses with carbazole


The optimum conditions for the calorimetric estimation of hexoses by reaction with carbazole in hot sulfuric acid solution have been determined and a convenient procedure, giving results with a precision of 2 to 5 per cent in the range of 50 to 150 γ of glucose, is described. The colors obtained with glucose, galactose, fructose, and mannose are not sufficiently distinctive to allow their ready differentiation and identification by spectral measurements. The significance of the ultraviolet spectra of heated and unheated sulfuric acid solutions of hexoses to the problem of estimation and identification of hexoses is discussed.

Additional Information

Copyright © 1947 by American Society of Biological Chemists. (Received for publication, July 16, 1947) Gates and Crellin Laboratories of Chemistry, Contribution No. 1137.

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