Combined optical and mechanical scanning in optical-resolution photoacoustic microscopy
Combined optical and mechanical scanning (COMS) in optical-resolution photoacoustic microscopy (OR-PAM) has provided five scanning modes with fast imaging speed and wide field of view (FOV). With two-dimensional (2D) galvanometer-based optical scanning, we have achieved a 2 KHz B-scan rate and 50 Hz volumetric-scan rate, which enables real-time tracking of cell activities in vivo. With optical-mechanical hybrid 2D scanning, we are able to image a wide FOV (10×8 mm2) within 150 seconds, which is 20 times faster than the conventional mechanical scan in our second-generation OR-PAM. With three-dimensional mechanical-based contour scanning, we can maintain the optimal signal-to-noise ratio and spatial resolution of OR-PAM while imaging objects with uneven surfaces, which is ideal for fast and quantitative studies of tumors and the brain.
Additional Information© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE). The authors appreciate Prof. James Ballard's close reading of the manuscript, and thank Jinyang Liang, Chiye Li, and Yong Zhou for helpful discussions and experimental assistance. This work was sponsored in part by National Institutes of Health (NIH) grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA159959, and R01 CA134539. L.V. Wang has a financial interest in Microphotoacoustics Inc., and Endra Inc., which, however, did not support this work. K. I. Maslov has a financial interest in Microphotoacoustics Inc.
Published - 89435X.pdf