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Published October 15, 2004 | public
Journal Article

Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics


We report on a silicon wafer-based device that can be used for recording macroscopic ion channel protein activities across a diverse group of cell-types. Gigaohm seals were achieved for CHO-K1 and RIN m5F cells, and both cell-attached and whole-cell mode configurations were also demonstrated. Two distinct intrinsic potassium ion channels were recorded in whole-cell mode for HIT-T15 and RAW 264.7 cells. Polydimethylsiloxane (PDMS) microfluidics were also coupled with the micromachined silicon chips in order to demonstrate that a single cell could be selectively directed to a micropore, and membrane protein currents could subsequently be recorded. These silicon chip-based devices have significant advantages over traditional micropipette approaches, and may serve as combinatorial tools for investigating membrane biophysics, pharmaceutical screening, and other bio-sensing tasks.

Additional Information

© 2004 Elsevier. Received 7 October 2003, Revised 15 February 2004, Accepted 25 February 2004, Available online 6 May 2004. This work was funded by the W.M. Keck Foundation, the Semiconductor Research Corporation, the NASA-funded CMISE center, and NIH grant Gm30376. We would like to thank B. Ribalet, and A. Aderem for supplying the RIN m5F, and RAW 264.7 cells, respectfully. We also acknowledge the UCLA Nanolab for assistance and advice in device fabrication.

Additional details

August 22, 2023
October 25, 2023