Long-Lived Engineering of Glycans to Direct Stem Cell Fate
Abstract
Glycans mediate many critical, long-term biological processes, such as stem cell differentiation. However, few methods are available for the sustained remodeling of cells with specific glycan structures. A new strategy that enables the long-lived presentation of defined glycosaminoglycans on cell surfaces using HaloTag proteins (HTPs) as anchors is reported. By controlling the sulfation patterns of heparan sulfate (HS) on pluripotent embryonic stem cell (ESC) membranes, it is demonstrated that specific glycans cause ESCs to undergo accelerated exit from self-renewal and differentiation into neuronal cell types. Thus, the stable display of glycans on HTP scaffolds provides a powerful, versatile means to direct key signaling events and biological outcomes such as stem cell fate.
Additional Information
© 2014 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: September 18, 2014; Revised: November 11, 2014. Article first published online: 4 Dec. 2014. This research was supported by a National Institutes of Health grant (R01-GM093627; L.H.W.) and a National Science Foundation Graduate Research Fellowship (DGE-1144469; M.E.G.). We thank Greg Miller for assistance with microarrays. We also thank Fred Tan, Elizabeth Jensen, and the Dervan laboratory for assistance with qRT-PCR and helpful discussions.Attached Files
Accepted Version - nihms-712902.pdf
Supplemental Material - anie_201409258_sm_miscellaneous_information.pdf
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Additional details
- PMCID
- PMC4533927
- Eprint ID
- 52796
- Resolver ID
- CaltechAUTHORS:20141215-080313928
- R01-GM093627
- NIH
- DGE-1144469
- NSF Graduate Research Fellowship
- Created
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2014-12-15Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field