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Published July 15, 2003 | Published
Journal Article Open

Fourier-domain low-coherence interferometry for light-scattering spectroscopy

Abstract

We present a novel method for obtaining depth-resolved spectra for determining scatterer size through elastic- scattering properties. Depth resolution is achieved with a white-light source in a Michelson interferometer with the mixed signal and reference fields dispersed by a spectrograph. The spectrum is Fourier transformed to yield the axial spatial cross correlation between the signal and reference fields with near 1 m m depth resolution. Spectral information is obtained by windowing to yield the scattering amplitude as a function of wave number. The technique is demonstrated by determination of the size of polystyrene microspheres in a subsurface layer with subwavelength accuracy. Application of the technique to probing the size of cell nuclei in living epithelial tissues is discussed.

Additional Information

© 2003 Optical Society of America Received February 10, 2003

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