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Published June 1985 | public
Journal Article

Human β-interferon gene expression is regulated by an inducible enhancer element


We have localized the regulatory sequence required for viral or poly([)-poly(C) activation of human β-interferon gene expression to a region located between −37 and −77 from the mRNA cap site. This sequence has the characteristics of an inducible enhancer element: it can act upstream or downstream of the β-interferon gene regardless of its orientation, and at distances up to approximately 1 kilobase from its normal location. Moreover, this element can confer inducibility on a heterologous promoter. Further analysis has identified a minimal regulatory element of 14 base pairs within this enhancer. Sequences closely related to this element are present five times within the 5′-flanking regions of both the α- and β-interferon genes. The number of these minimal regulatory elements required for maximal β-interferon gene expression appears to differ in different cell lines.

Additional Information

© 1985 by MIT. Received March 25, 1985. We thank Tamar Enoch, Julian Banerji, Rick Myers, and Richard Treisman for stimulating discussions and helpful comments on the manuscript; T. Taniguchi for communicating results prior to publication; S. McKnight for providing the tk promoter mutants. S. G. was supported by a Travelling Fellowship from the Medical Research Council of Great Britain, and more recently by fellowship DRG-810 of the Damon Runyon-Walter Winchell Cancer Fund. This work was supported by a grant to T. M. from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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