Directing Neuronal Signaling through Cell-Surface Glycan Engineering
Abstract
The ability to tailor plasma membranes with specific glycans may enable the control of signaling events that are critical for proper development and function. We report a method to modify cell surfaces with specific sulfated chondroitin sulfate (CS) glycosaminoglycans using chemically modified liposomes. Neurons engineered to display CS-E-enriched polysaccharides exhibited increased activation of neurotrophin-mediated signaling pathways and enhanced axonal growth. This approach provides a facile, general route to tailor cell membranes with biologically active glycans and demonstrates the potential to direct important cellular events through cell-surface glycan engineering.
Additional Information
© 2014 American Chemical Society. ACS AuthorChoice. Received: January 17, 2014; publication Date (Web): April 18, 2014. We thank Rochelle Diamond at the Caltech Flow Cytometry Facility for flow cytometry expertise, Carol Garland for TEM imaging, and Jennifer Keeffe and Dorothy Pan for DLS and zeta potential analyses aid, respectively. This work was supported by a grant from the NIH (R01 GM093627) and an NSF GRF (M.E.G., DGE-1144469).Attached Files
Published - ja5005174.pdf
Supplemental Material - ja5005174_si_001.pdf
Files
Name | Size | Download all |
---|---|---|
md5:83b69a13fef90ba388113854d85a4637
|
10.0 MB | Preview Download |
md5:a05728ec1e42976b573f89075c4f94fc
|
956.4 kB | Preview Download |
Additional details
- PMCID
- PMC4120997
- Eprint ID
- 45478
- Resolver ID
- CaltechAUTHORS:20140505-091717887
- R01 GM093627
- NIH
- DGE-1144469
- NSF Graduate Research Fellowship
- Created
-
2014-05-05Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field