Random-access optical-resolution photoacoustic microscopy using a digital micromirror device

Creators: Liang, Jinyang; Zhou, Yong; Winkler, Amy W.; Wang, Lidai; Maslov, Konstantin I.; Li, Chiye; Wang, Lihong V.

Abstract

We developed random-access optical-resolution photoacoustic microscopy using a digital micromirror device. This system can rapidly scan arbitrarily shaped regions of interest within a 40 μm×40 μm imaging area with a lateral resolution of 3.6 μm. To identify a region of interest, a global structural image is first acquired, then the selected region is scanned. The random-access ability was demonstrated by imaging two static samples, a carbon fiber cross and a monolayer of red blood cells, with an acquisition rate up to 4 kHz. The system was then used to monitor blood flow in vivo in real time within user-selected capillaries in a mouse ear. By imaging only the capillary of interest, the frame rate was increased by up to 9.2 times.

Additional Information

© 2013 Optical Society of America. Received May 6, 2013; revised June 25, 2013; accepted June 27, 2013; posted June 28, 2013 (Doc. ID 190043); published July 22, 2013. The authors thank the help of Dr. Liang Gao in the system design, and Professor James Ballard's close reading of the manuscript. This work was sponsored in part by National Institutes of Health grants DP1 EB016986 (NIH Director's Pioneer Award), R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, R01 CA157277, and R01 CA159959. L. W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work. K. M. has a financial interest in Microphotoacoustics, Inc., which, however, did not support this work.

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