Wide field-of-view Talbot grid-based microscopy for multicolor fluorescence imaging
Abstract
The capability to perform multicolor, wide field-of-view (FOV) fluorescence microscopy imaging is important in screening and pathology applications. We developed a microscopic slide-imaging system that can achieve multicolor, wide FOV, fluorescence imaging based on the Talbot effect. In this system, a light-spot grid generated by the Talbot effect illuminates the sample. By tilting the excitation beam, the Talbot-focused spot scans across the sample. The images are reconstructed by collecting the fluorescence emissions that correspond to each focused spot with a relay optics arrangement. The prototype system achieved an FOV of 12 × 10 mm^2 at an acquisition time as fast as 23 s for one fluorescence channel. The resolution is fundamentally limited by spot size, with a demonstrated full-width at half-maximum spot diameter of 1.2 μm. The prototype was used to nimage green fluorescent beads, double-stained human breast cancer SK-BR-3 cells, Giardia lamblia cysts, and the Cryptosporidium parvum oocysts. This imaging method is scalable and simple for implementation of high-speed wide FOV fluorescence microscopy.
Additional Information
© 2013 Optical Society of America. Received 14 May 2013; revised 5 Jun 2013; accepted 5 Jun 2013; published 11 Jun 2013. We thank Hao Yuan Kueh (Division of Biology, Caltech) for helpful discussions on large-FOV imaging based on conventional microscopy. This project was funded by the NIH under grant 1R01AI096226-01.Attached Files
Published - oe-21-12-14555.pdf
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Additional details
- PMCID
- PMC3726246
- Eprint ID
- 39762
- Resolver ID
- CaltechAUTHORS:20130805-103927291
- NIH
- 1R01AI096226-01
- Created
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2013-08-05Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field