Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published June 17, 2013 | Published
Journal Article Open

Wide field-of-view Talbot grid-based microscopy for multicolor fluorescence imaging

Abstract

The capability to perform multicolor, wide field-of-view (FOV) fluorescence microscopy imaging is important in screening and pathology applications. We developed a microscopic slide-imaging system that can achieve multicolor, wide FOV, fluorescence imaging based on the Talbot effect. In this system, a light-spot grid generated by the Talbot effect illuminates the sample. By tilting the excitation beam, the Talbot-focused spot scans across the sample. The images are reconstructed by collecting the fluorescence emissions that correspond to each focused spot with a relay optics arrangement. The prototype system achieved an FOV of 12 × 10 mm^2 at an acquisition time as fast as 23 s for one fluorescence channel. The resolution is fundamentally limited by spot size, with a demonstrated full-width at half-maximum spot diameter of 1.2 μm. The prototype was used to nimage green fluorescent beads, double-stained human breast cancer SK-BR-3 cells, Giardia lamblia cysts, and the Cryptosporidium parvum oocysts. This imaging method is scalable and simple for implementation of high-speed wide FOV fluorescence microscopy.

Additional Information

© 2013 Optical Society of America. Received 14 May 2013; revised 5 Jun 2013; accepted 5 Jun 2013; published 11 Jun 2013. We thank Hao Yuan Kueh (Division of Biology, Caltech) for helpful discussions on large-FOV imaging based on conventional microscopy. This project was funded by the NIH under grant 1R01AI096226-01.

Attached Files

Published - oe-21-12-14555.pdf

Files

oe-21-12-14555.pdf
Files (2.2 MB)
Name Size Download all
md5:459a0528df9e5a2610b063d134ddb92f
2.2 MB Preview Download

Additional details

Created:
August 19, 2023
Modified:
October 24, 2023