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Published December 12, 2007 | Supplemental Material + Published
Journal Article Open

Interactions between the NR2B receptor and CaMKII modulate synaptic plasticity and spatial learning.


The NR2B subunit of the NMDA receptor interacts with several prominent proteins in the postsynaptic density, including calcium/calmodulin-dependent protein kinase II (CaMKII). To determine the function of these interactions, we derived transgenic mice expressing a ligand-activated carboxy-terminal NR2B fragment (cNR2B) by fusing this fragment to a tamoxifen (TAM)-dependent mutant of the estrogen receptor ligand-binding domain LBD(G521R). Here, we show that induction by TAM allows the transgenic cNR2B fragment to bind to endogenous CaMKII in neurons. Activation of the LBD(G521R)-cNR2B transgenic protein in mice leads to the disruption of CaMKII/NR2B interactions at synapses. The disruption decreases Thr286 phosphorylation of alphaCaMKII, lowers phosphorylation of a key CaMKII substrate in the postsynaptic membrane (AMPA receptor subunit glutamate receptor 1), and produces deficits in hippocampal long-term potentiation and spatial learning. Together our results demonstrate the importance of interactions between CaMKII and NR2B for CaMKII activity, synaptic plasticity, and learning.

Additional Information

© 2007 Society for Neuroscience. Received July 30, 2007; revised Oct. 25, 2007; accepted Oct. 25, 2007. This work was supported by National Institutes of Health Grant 98-069-23 (A.J.S.). We thank Robert A. M. Brown and Anna Matynia for critical discussions and helpful comments, and Edoardo Dado Marcora and Tinh Luong for expert technical assistance.

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Supplemental Material - Supplemental_Legend.pdf

Supplemental Material - supplemental_figure_1.gif

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August 22, 2023
August 22, 2023