CaltechAUTHORS
Published October 4, 2010 | Version Accepted Version + Supplemental Material
Journal Article Open

Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells

Creators

  • 1. ROR icon California Institute of Technology
  • 2. ROR icon Korea Advanced Institute of Science and Technology
  • 3. ROR icon Yale University

Abstract

The optimization of chemistries to enable the patterning of miniaturized DNA barcodes using microfluidics flow channels is described (see picture). Experiment and theory reveal that solvent mixtures in which counterions are strongly associated with the negatively charged DNA oligomers may be harnessed to produce high quality, high density DNA microarray patterns over a large area.

Additional Information

© 2010 Wiley-VCH Verlag GmbH& Co. KGaA, Weinheim. Received: July 1, 2010. Published online on August 16, 2010. This work was supported by National Cancer Institute Grant No. 5U54 CA119347 (J.R.H., P.I.) and by a gift from the Jean Perkins Foundation. H. K, T. A. P and W. A. G also acknowledge support from the WCU programs through NRF of Korea funded by the MEST (R31–2008–000–10055–0).

Attached Files

Accepted Version - nihms478904.pdf

Supplemental Material - cphc_201000528_sm_miscellaneous_information.pdf

Files

cphc_201000528_sm_miscellaneous_information.pdf

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Additional details

Identifiers

PMCID
PMC3681607
Eprint ID
20721
Resolver ID
CaltechAUTHORS:20101108-150340183

Funding

NIH
5U54 CA119347
National Research Foundation of Korea
R31-2008-000-10055-0
Jean Perkins Foundation
National Cancer Institute

Dates

Created
2010-11-29
Created from EPrint's datestamp field
Updated
2021-11-09
Created from EPrint's last_modified field