Realtime photoacoustic microscopy of murine cardiovascular and respiratory dynamics in vivo
While photoacoustic imaging has emerged as a promising modality in recent years, a key drawback of practical and widespread use of the technique has been slow imaging rates. We present a 30-MHz array-based photoacoustic imaging system that can acquire and display photoacoustic images in realtime. Realtime display is very helpful and provides the system operator the ability to better navigate and position the probe for selecting a desired anatomical field of view. The system is capable of imaging at 50 frames per second to depths of a few mm in tissue. We used this system to successfully image the beating hearts of young athymic nude mice in vivo. Also of interest was the ability to visualize microvascular changes during respiration.
Additional Information© 2008 Society of Photo-Optical Instrumentation Engineers (SPIE). We gratefully acknowledge helpful discussions with Dr. William D. Richard, who suggested the use of the dual-socket quad-core computers, and Dr. Robert E Morley Jr. and Mr. Ed Richter for allowing us to test the feasibility of our realtime beamforming algorithm on their dual-socket quad core workstations. We thank Dr. George Stoica for assistance with animal protocols. This work was funded in part by grants R01 EB000712 and R01 NS46214 from National Institutes of Health.
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