Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published November 12, 2007 | Accepted Version + Supplemental Material
Journal Article Open

DNA Strand Cleavage near a CC Mismatch Directed by a Metalloinsertor


Reagents for recognition and efficient cleavage of mismatched DNA without photoactivation were designed. They contain a combination of a mismatch-directing metalloinsertor, [Rh(bpy)_2(chrysi)]^(3+) (bpy = 2,2'-bipyridyl, chrysi = 5,6-chrysenequinone diimine), and an oxidative cleavage functionality, [Cu(phen)_2]^+ (Cu). Both unconjugated (Rh+Cu) and conjugated (Rh−Cu) frameworks of the Rh insertor and Cu were prepared. Compared to Cu, both constructs Rh+Cu and Rh−Cu exhibit efficient site-specific DNA scission only with mismatched DNA, confirmed by experiments with ^(32)P-labeled oligonucleotides. Furthermore, these studies indicate that DNA cleavage occurs near the mismatch in the minor groove and on both strands. Interestingly, the order of reactivity of the three systems with a CC mismatch is Rh+Cu > Rh−Cu ≫ Cu. Rh binding appears to direct Cu reactivity with or without tethering. These results illustrate advantages and disadvantages in bifunctional conjugation.

Additional Information

© 2007 American Chemical Society. Received August 10, 2007. Publication Date (Web): October 6, 2007. We are grateful to the NIH (Grant GM33309) and Applied Biosystems for their financial support. We also thank the Tobacco-Related Disease Research Program (TRDRP) for a postdoctoral fellowship to M.H.L.

Attached Files

Accepted Version - nihms122811.pdf

Supplemental Material - ic701598k_si_002.pdf


Files (939.0 kB)
Name Size Download all
746.0 kB Preview Download
193.0 kB Preview Download

Additional details

August 19, 2023
October 17, 2023