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Published August 2012 | Accepted Version
Journal Article Open

A Multimode Optical Imaging System for Preclinical Applications In Vivo: Technology Development, Multiscale Imaging, and Chemotherapy Assessment


Purpose: Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging. Procedures: We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models. Results: The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment. Conclusions: This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.

Additional Information

© 2011 World Molecular Imaging Society. Published Online: 27 August 2011. We thank Dr. Mark Gaon for help developing and testing the gated anesthesia instrument. Some of this work was done in partial fulfillment of Ph.D. thesis research requirements by Dr. J.Y. Hwang, at the University of Southern California. Work at the California Institute of Technology was supported by the Arnold and Mabel Beckman Foundation. Z.G. thanks Johnson & Johnson for research support. We are grateful for the following federal support of our research: NIH (5R01CA123495-03 and 1U01CA151815-0) to JYL; NIH (1R01 CA140995 and 1R01 CA129822) and DOD W81XWH-06-1-0549 to LKMK; and US Navy Bureau of Medicine and Surgery (1435-04-04-GT-41387 and -43096), NIH (N01-CO- 07119), and NSF (BESOO 79483) to DLF. Conflict of Interest. The authors declare they have no conflicts of interest.

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August 22, 2023
October 18, 2023