Detection of c-sis Transcripts and Synthesis of PDGF-Like Proteins by Human Osteosarcoma Cells

Creators: Graves, D. T.; Owen, A. J.; Barth, R. K.; Tempst, P.; Winoto, A.; Fors, L.; Hood, L. E.; Antoniades, H. N.

Abstract

Platelet-derived growth factor (PDGF) has been previously shown to be homologous to the transforming gene of simian sarcoma virus (v-sis), and inappropriate expression of the cellular counterpart of the v-sis gene (c-sis) has been implicated in the generation of mesenchymal tumors. The U-2 OS human osteosarcoma line was shown to contain multiple c-sis transcripts. Immunoprecipitation experiments with antiserum to PDGF identified a variety of polypeptides ranging in size from 18,000 to 165,000 daltons that were immunoprecipitated specifically from U-2 OS cell extracts. The osteosarcoma also was shown to secrete a 29,000-dalton protein having the serological and structural characteristics of PDGF.

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© 1984 American Association for the Advancement of Science. 18 May 1984; Accepted 27 July 1984. We thank S. Williams, R. Siraco, G. Easterly, C. Katz, and J. MacLaren for technical assistance. Supported by National Cancer Institute grant CA30101 (H.N.A.) and NIH grants HL29583 (H.N.A.) and HL27607 (A.J.O.). R.K.B. is a fellow of the Anna Fuller Fund and P.T. is a special fellow of the Leukemia Society of America.

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Detection of c-sis Transcripts and Synthesis of PDGF-Like Proteins by Human Osteosarcoma Cells

Abstract

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