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Published January 15, 2010 | public
Journal Article

Internally calibrated quantification of VEGF in human plasma by fluorescence immunoassays in disposable elastomeric microfluidic devices


Herein we report on a proof of principle for the reproducible quantification of Vascular Endothelial Growth Factor (VEGF) in human plasma by fluorescence sandwich immunoassays using disposable polydimethylsiloxane (PDMS) microfluidic chips. The system requires 100 times less sample than typical clinical blood tests, while its current quantification limit is established at 4 pM. The in-built calibration method of spiking the plasma with known concentrations of commercially available antigen avoids common sources of error and improves the reliability of the test results. The demonstrated technique is important for immunoassay applications in fundamental scientific research and "point-of-care" (POC) biomedical diagnostics. In particular, the system is immediately applicable to microfluidic quantification of VEGF in human plasma in cancer studies.

Additional Information

© 2009 Elsevier. Received 19 May 2009; accepted 25 August 2009. Available online 29 August 2009. The authors thank the USC Reference Laboratory for providing the plasma samples. The authors also thank Caltech Kavli Nanoscience Institute and the Caltech Microfluidics Foundry for providing access to their nanofabrication facilities. Financial support was provided by NIH grant R01-HG0026440, NIH grant 1K99007151, NIH grant 4R00EB007151-03, and the Caltech Summer Undergraduate Research Fellowship (SURF) program.

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