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Published August 28, 2012 | public
Book Section - Chapter

Three-Dimensional Optical-Resolution Photoacoustic Microscopy

Abstract

Three-dimensional optical-resolution photoacoustic microscopy (OR-PAM), an emerging optical-acoustic hybrid technology capable of imaging optical absorption contrasts with subcellular resolution and sensitivity, has been recognized as a valuable complement to existing optical microscopy technologies. In this chapter, we provide detailed discussion on the design and operation of OR-PAM, including the principle, system design, system configuration, system alignment, experimental procedures, laser safety, functional imaging scheme, recent technical advances, and sample biomedical applications. Future directions of OR-PAM development are also discussed at the end of this chapter.

Additional Information

© 2013 Springer-Verlag Berlin Heidelberg. This work was sponsored by National Institutes of Health Grants R01 EB000712, EB000712A2S1, R01 EB00071207S2, R01 EB008085, R01 CA113453901, U54 CA136398, and 5P60 DK02057933. Professor Lihong V. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

Additional details

Created:
August 19, 2023
Modified:
October 19, 2023