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Published December 27, 1996 | public
Journal Article

Subregion- and Cell Type–Restricted Gene Knockout in Mouse Brain


Using the phage P1–derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination–based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

Additional Information

© 1996 by Cell Press. Received 8 November 1996, Revised 25 November 1996, Available online 3 October 2000. We wish to thank Jason Derwin for technical help and Emily Rossie and Pamela Woronoff for secretarial help. We are grateful to Brian Sauer for the gift of plasmid pBS317 and to Nobuki Nakanishi for the gift of plasmid pNN265. This work was supported by National Institutes of Health grant NS32925 and by gifts from the Shionogi Institute for Medical Science and Amgen, Inc. (all to S.T.).

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