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Published February 23, 2010 | Published
Book Section - Chapter Open

In vivo, label-free photoacoustic microscopy of the anterior segment of the mouse eye


Both iris fluorescein angiography (IFA) and indocyanine green angiography (ICGA) provide ophthalmologists imaging tools in studying the microvasculature structure and hemodynamics of the anterior segment of the eye in normal and diseased status. However, a non-invasive, endogenous imaging modality is preferable for the monitoring of hemodynamics of the iris microvasculature. We investigated the in vivo, label-free ocular anterior segment imaging with photo-acoustic microscopy (PAM) in mouse eyes. We demonstrated the unique advantage of endogenous contrast that is not available in both IFA and ICGA. The laser radiation was maintained within the ANSI laser safety limit. The in vivo, label-free nature of our imaging technology has the potential for ophthalmic applications.

Additional Information

© 2010 Society of Photo-Optical Instrumentation Engineers (SPIE). The mouse ocular anterior segment imaging demonstration done on the original OR-PAM system was contributed in equal measure by Bin Rao and Song Hu. The HS-ORPAM system and mouse ocular anterior segment imaging demonstration were contributed in equal measure by Bin Rao and Li Li. The research was supported by the National Institutes of Health grants R01 EB000712, EB000712A2S1, R01 EB00071207S2, R01 EB008085, R01 CA113453901, U54 CA136398, and 5P60 DK02057933. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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