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Published May 15, 2010 | Supplemental Material
Journal Article Open

Hybrid-scanning optical-resolution photoacoustic microscopy for in vivo vasculature imaging


Recently developed optical-resolution photoacoustic microscopy (OR-PAM), which is based on the detection of optical absorption contrast, is complementary to other optical microscopy modalities such as optical confocal microscopy, optical coherence tomography, and multiphoton microscopy. A hybrid optical–mechanical scanning configuration increases the imaging speed of OR-PAM significantly, facilitating many demanding biomedical applications. With a high-pulse-repetition-rate laser, the hybrid-scanning OR-PAM can acquire one-dimensional depth-resolved images (A-lines) at 5 kHz and two-dimensional B-scan images containing 800 A-lines at 6.25 Hz. We demonstrated in vivo in a mouse three-dimensional imaging of the iris vasculature in 128 s for an 800×800×200 data set and of the ear vasculature in 256 s for an 800×1600×200 data set.

Additional Information

© 2010 Optical Society of America. Received February 11, 2010; revised March 24, 2010; accepted March 31, 2010; posted April 6, 2010 (Doc. ID 124078); published May 5, 2010. The research was supported by the National Institutes of Health (NIH) grants R01 EB000712, EB000712A2S1, R01 EB00071207S2, R01 EB008085, R01 CA113453901, U54 CA136398, and 5P60 DK02057933. L.W. has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which, however, did not support this work.

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