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Published February 24, 2009 | Published
Book Section - Chapter Open

Photoacoustic microscopy of cerebral blood-oxygenation dynamics in mice


In this work, we exploit the high depth and temporal resolutions of PAM to noninvasively image the blood-oxygenation dynamics of multiple cortex vessels in the mouse brain simultaneously in response to controlled hypoxic and hyperoxic challenges. The dark-field photoacoustic microscopy (PAM) technique was enhanced to image the cortex vasculature of the mouse brain in vivo using endogenous hemoglobin contrast with one second temporal resolution. The maximum values of about 20% with standard deviation ± 1.2% were found to vary significantly among the cortex vessels studied. The hypoxic response time to rise from 10 % to 90 % of maximum was 63 ± 6 sec. The reverse response time for this event was 16 ± 2 sec.

Additional Information

© 2009 Society of Photo-Optical Instrumentation Engineers (SPIE). This project is sponsored in part by National Institutes of Health Grant Nos. R01 EB000712, R01 NS46214 (BRP), R01 EB008085 and U54 CA136398 (Network for Translational Research). L.W. has a financial interest in Endra, Inc., which, however, did not support this work.

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