Fast 3-D photoacoustic imaging in vivo with a high frequency ultrasound array toward clinical applications
We present an in vivo reflection-mode photoacoustic microscopy system that performs B-scan imaging at 50 Hz with realtime beamforming and 3-D imaging of 166 B-scan frames at 1 Hz with post-beamforming. To our knowledge, this speed is currently the fastest in high frequency photoacoustic imaging. In addition, with a custom fiber based light delivery system, the imaging device is capable of performing handheld operation. Software for image processing and display with clinically user-friendly graphic user interface (GUI) is developed. The system has axial, lateral, and elevational resolutions of 25, 70, and 200 μm, respectively, and can image 3 mm deep in scattering biological tissue. Volumetric images of subcutaneous vasculature in murine are demonstrated in vivo. The system is anticipated to have potential clinical applications in skin melanoma detection due to its unique ability to image in realtime and to image anatomical sites inaccessible to other imaging systems.
Additional Information© 2009 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was sponsored by grants R01 EB000712, R01 EB008085, R01 NS46214, and U54 CA136398 (Network for Translational Research) from the National Institutes of Health. L.W. has a financial interest in Endra, Inc., which, however, did not support this work.
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