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Published August 1, 1988 | Published
Journal Article Open

A-Type Potassium Channels Expressed from Shaker Locus cDNA


A-type K+ currents are expressed in Xenopus oocytes injected with in vitro-synthesized transcripts from cDNAs for the Drosophila Shaker (Sh) locus. A single Sh gene product, possibly as a multimer, is sufficient for formation of functional A channels. Various Sh RNAs express A currents with distinct kinetic properties. An analysis of structure-function relationships shows that the conserved central region of Sh polypeptides determines ionic selectivity and overall channel behavior, whereas the divergent amino and carboxyl termini can modify channel kinetics. Alternative splicing of Sh gene transcripts may provide one mechanism for the generation of K+ channel diversity.

Additional Information

© 1988 by the National Academy of Sciences Contributed by N. Davidson, April 1, 1988 We thank D. Krafte for his expert advice in oocyte patch clamping, and J. Campanelli, A. Kamb, M. Mathew, J.D. Pollock, and M. Ramaswani for their comments. This research was supported by U.S. Public Health Service Grants GM26976 to B.R.; NS21327 to M.A.T., GM10991 to N.D., NS11756 to H.A.L., and a Pfeiffer Research Foundation grant to M.A.T. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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