Mosaic sarbecovirus vaccination elicits cross-reactive responses in pre-immunized animals

We thank Jesse Bloom (Fred Hutchinson) and Tyler Starr (University of Utah) for RBD libraries and help setting up DMS at Caltech, Noor Youssef (Harvard Medical School) and Ziyan Wu (Caltech) for radar plot Python scripts, Jost Vielmetter, Luisa Segovia, Annie Lam, and the Caltech Beckman Institute Protein Expression Center for protein production, Igor Antoshechkin and the Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory for Illumina sequencing, S. Alison Arnold, Connor Bowen, Sharon Versteeg, Jack E. Kay, Joseph Moores-Poole, and Vasilis Tsakonitis (Ingenza, Ltd.) for expressing and purifying Bacillus mi3 and Pichia RBDs, Marta Murphy for help making figures, the Viral Vector Core Facility (Oxford University) for ChAdOx1 nCoV-19, Helix Biotech for Pfizer-like WA1 and BA.5 mRNA-LNP, Jeffrey Ravetch (Rockefeller University) for the Moderna WA1 mRNA-LNP vaccine, Labcorp Drug Development (Denver, PA) for performing BALB/c mouse immunizations for the mRNA-LNP and ChAdOx1 nCoV-19 experiments, T. Kurosaki and T. Okada (U. Osaka, RIKEN-Yokohama) for S1pr2-Cre^ERT2 BAC-transgenic mice, and Alain Townsend and Jack T. K. Tan (Oxford), Andrew DeLaitsch and Magnus Hoffmann (Caltech), and Bjorkman lab members for helpful discussions. These studies were funded by Wellcome Leap (P.J.B.), the National Institutes of Health: [P01-AI165075 (P.J.B.), R01AI119006 and R01AI173086 (G.D.V.), R01AI153064 (N.P.)], Bill and Melinda Gates Foundation INV-034638 (P.J.B.), the Coalition for Epidemic Preparedness Innovations (CEPI) (P.J.B., I.F.), the Merkin Institute for Translational Research (Caltech), and the Stavros Niarchos Foundation Institute (Rockefeller).