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Published April 1, 2011 | Published
Journal Article Open

Second-generation optical-resolution photoacoustic microscopy with improved sensitivity and speed


We developed second-generation (G2) optical-resolution photoacoustic microscopy (OR-PAM). Incorporation of a novel acoustic detection scheme improved upon the sensitivity of our first-generation (G1) system by 18.4 dB, deepening the in vivo tissue penetration to 1.2 mm at 570 nm. Moreover, translating the imaging head instead of the living object accelerated the scanning speed by a factor of 5, widening the field of view within the same acquisition time. Mouse ears, as well as mouse brains with intact craniums, were imaged in vivo in both total concentration and oxygen saturation of hemoglobin.

Additional Information

© 2011 Optical Society of America. Received December 21, 2010; accepted February 3, 2011; posted February 16, 2011 (Doc. ID 139968); published March 23, 2011. The authors appreciate James Ballard's close reading of the manuscript, and thank Ernie Gonzales and Christopher Favazza for experimental assistance. This work was sponsored by National Institutes of Health (NIH) grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, and 5P60 DK02057933. Lihong Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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