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Published April 10, 2003 | Supplemental Material
Journal Article Open

SOX10 Maintains Multipotency and Inhibits Neuronal Differentiation of Neural Crest Stem Cells


The mechanisms that establish and maintain the multipotency of stem cells are poorly understood. In neural crest stem cells (NCSCs), the HMG-box factor SOX10 preserves not only glial, but surprisingly, also neuronal potential from extinction by lineage commitment signals. The latter function is reflected in the requirement of SOX10 in vivo for induction of MASH1 and PHOX2B, two neurogenic transcription factors. Simultaneously, SOX10 inhibits or delays overt neuronal differentiation, both in vitro and in vivo. However, this activity requires a higher Sox10 gene dosage than does the maintenance of neurogenic potential. The opponent functions of SOX10 to maintain neural lineage potentials, while simultaneously serving to inhibit or delay neuronal differentiation, suggest that it functions in stem or progenitor cell maintenance, in addition to its established role in peripheral gliogenesis.

Additional Information

© 2003 Cell Press. Under an Elsevier user license. Received 16 September 2002; Revised 3 March 2003, Accepted 7 March 2003, Available online 17 April 2004; Published: April 9, 2003. We thank Carmen Birchmeier for support and helpful discussions, Stefan Britsch for providing genotyped Dom embryos, Mark Marcchionni for his generous gift of recombinant GGFII/NRG-1, Bill Pavan for the SOX10 cDNA clone, and J.-F. Brunet and C. Goridis for PHOX2A/B reagents. We thank Gaby Mosconi for laboratory management, N. Shah, S. Lowell, and Q. Zhou for critical comments on the manuscript, and members of the Anderson laboratory for helpful discussions. J.K. is an Associate and D.J.A. an Investigator of the Howard Hughes Medical Institute.

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