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Published June 4, 2014 | Version public
Book Section - Chapter Metadata-only

Extraction and Measurement of NAD(P)+ and NAD(P)H

Creators

  • 1. ROR icon Massachusetts Institute of Technology
  • 2. ROR icon California Institute of Technology
  • 3. ROR icon Columbia University

Contributors

Abstract

Nicotinamide adenine dinucleotides are critical redox-active substrates for countless catabolic and anabolic reactions. Ratios of NAD+ to NADH and NADP+ to NADPH are therefore considered key indicators of the overall intracellular redox potential and metabolic state. These ratios can be measured in bulk conditions using a highly sensitive enzyme cycling-based colorimetric assay (detection limit at or below 0.05 μM or 1 pmol) following a simple extraction procedure involving solutions of acid and base. Special considerations are necessary to avoid measurement artifacts caused by the presence of endogenous redox-active metabolites, such as phenazines made by diverse Pseudomonas species (see Chapter 25).

Additional Information

Ⓒ 2014 Springer Science+Business Media New York.

Additional details

Identifiers

Eprint ID
77593
Resolver ID
CaltechAUTHORS:20170518-165224584

Dates

Created
2017-05-19
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Updated
2021-11-15
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Caltech Custom Metadata

Caltech groups
Division of Geological and Planetary Sciences (GPS)
Series Name
Methods in Molecular Biology
Series Volume or Issue Number
1149