Postsynthetic Photocontrol of Giant Liposomes via Fusion-Based Photolipid Doping
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1.
New York University
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2.
University of California, San Francisco
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3.
California Institute of Technology
Abstract
We report on photolipid doping of giant unilamellar vesicles (GUVs) via vesicle fusion with small unilamellar photolipid vesicles (pSUVs), which enables retroactive optical control of the membrane properties. We observe that vesicle fusion is light-dependent, if the phospholipids are neutral. Charge-mediated fusion involving anionic and cationic lipid molecules augments the overall fusion performance and doping efficiency, even in the absence of light exposure. Using phosphatidylcholine analogs with one or two azobenzene photoswitches (azo-PC and dazo-PC) affects domain formation, bending stiffness, and shape of the resulting vesicles in response to irradiation. Moreover, we show that optical membrane control can be extended to long wavelengths using red-absorbing photolipids (red-azo-PC). Combined, our findings present an attractive and practical method for the precise delivery of photolipids, which offers new prospects for the optical control of membrane function.
Copyright and License
This publication is licensed under CC-BY 4.0 . Copyright © 2022 The Authors. Published by American Chemical Society
Acknowledgement
This work has been supported by the Deutsche Forschungsgemeinschaft (DFG) through the Collaborative Research Center (SFB1032, Project no. 201269156, projects A8 and B9), the Transregional Collaborative Research Center (TRR152, Project no. 239283807, project P26), and by the European Research Council Consolidator Grant "ProForce" . The authors thank local research clusters and centers such as the Center of Nanoscience (CeNS) for providing communicative networking structures. J.M. thanks the German Academic Scholarship Foundation for a fellowship, the New York University for a MacCracken fellowship and a Margaret and Herman Sokol fellowship, and the NCI for an F99/K00 award (K00CA253758).
Conflict of Interest
The authors declare no competing financial interest.
Additional Information
The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.langmuir.2c01685.
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Additional information on the synthetic procedures, materials, methods, and analytical/supporting data (PDF)
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Domains inazo-PC-doped GUV (Movie S1) (AVI)
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Photo-triggered membrane fluctuations and elongation of dazo-PC-doped GUV (Movie S2) (AVI)
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UV-A-triggered budding event of dazo-PC-doped GUV (Movie S3) (AVI)
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Back-switching (cis-to-trans) of dazo-PC-doped GUV/reverse of the shape transition (budding event) (Movie S4) (AVI)
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Photo-triggered membranefluctuations of red-azo-PC-doped GUV (Movie S5) (AVI)
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Additional details
- National Cancer Institute
- K00CA253758
- Deutsche Forschungsgemeinschaft
- SFB1032
- Deutsche Forschungsgemeinschaft
- TRR152
- New York University
- Publication Status
- Published