Prokineticin 2 Is a Target Gene of Proneural Basic Helix-Loop-Helix Factors for Olfactory Bulb Neurogenesis

Creators: Zhang, Chengkang; Ng, Kwan L.; Li, Jia-Da; He, Fei; Anderson, David J.; Sun, Yi E.; Zhou, Qun-Yong

Abstract

Prokineticin 2, a cysteine-rich secreted protein, regulates diverse biological functions including the neurogenesis of olfactory bulb. Here we show that the PK2 gene is a functional target gene of proneural basic helix-loop-helix (bHLH) factors. Neurogenin 1 and MASH1 activate PK2 transcription by binding to E-box motifs on the PK2 promoter with the same set of E-boxes critical for another pair of bHLH factors, CLOCK and BMAL1, in the regulation of circadian clock. Our results establish PK2 as a common functional target gene for different bHLH transcriptional factors in mediating their respective functions.

Additional Information

© 2007 the American Society for Biochemistry and Molecular Biology. Received for publication, November 15, 2006 , and in revised form, January 17, 2007. We thank Dr. Michael Greenberg for providing anti-Ngn1 antibodies. [K.L.N. was a] recipient of a University of California Irvine Medical Scientist Training Program training grant. The on-line version of this article (available at http://www.jbc.org) contains four supplemental figures. The work was supported in part by a University of California Discovery grant. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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