Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivo
Abstract
We have demonstrated the feasibility of imaging mouse brain metabolism using photoacoustic computed tomography (PACT), a fast, noninvasive and functional imaging modality with optical contrast and acoustic resolution. Brain responses to forepaw stimulations were imaged transdermally and transcranially. 2-NBDG, which diffuses well across the blood–brain-barrier, provided exogenous contrast for photoacoustic imaging of glucose response. Concurrently, hemoglobin provided endogenous contrast for photoacoustic imaging of hemodynamic response. Glucose and hemodynamic responses were quantitatively decoupled by using two-wavelength measurements. We found that glucose uptake and blood perfusion around the somatosensory region of the contralateral hemisphere were both increased by stimulations, indicating elevated neuron activity. While the glucose response area was more homogenous and confined within the somatosensory region, the hemodynamic response area had a clear vascular pattern and spread wider than the somatosensory region. Our results demonstrate that 2-NBDG-enhanced PACT is a promising tool for noninvasive studies of brain metabolism.
Additional Information
© 2012 Elsevier Inc. Accepted 15 August 2012; Available online 24 August 2012. The authors appreciate Prof. James Ballard's close reading of the manuscript, and thank Arie Krumholz, Muhammad Chatni and Lidai Wang for their useful discussion and technical assistance. This work was sponsored by the National Institutes of Health (NIH) grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, R01 CA157277, and R01 CA159959. Lihong Wang has a financial interest in Endra, Inc., which, however, did not support this work. Lihong Wang and K. Maslov have a financial interest in Microphotoacoustics, Inc., which did not support this work.Attached Files
Accepted Version - nihms-402470.pdf
Files
Name | Size | Download all |
---|---|---|
md5:2966227658d2de844f1f505d13449a5a
|
2.6 MB | Preview Download |
Additional details
- PMCID
- PMC3508393
- Eprint ID
- 68971
- Resolver ID
- CaltechAUTHORS:20160711-151157171
- NIH
- R01 EB000712
- NIH
- R01 EB008085
- NIH
- R01 CA134539
- NIH
- U54 CA136398
- NIH
- R01 EB010049
- NIH
- R01 CA157277
- NIH
- R01 CA159959
- Created
-
2016-07-27Created from EPrint's datestamp field
- Updated
-
2021-11-11Created from EPrint's last_modified field