Molecular cloning and characterization of a new member of the gap junction gene family, connexin-31
- Creators
- Hoh, Jan H.
- John, Scott A.
- Revel, Jean-Paul
Abstract
A new member of the connexin gene family has been identified and designated rat connexin-31 (Cx31) based on its predicted molecular mass of 30,960 daltons. Cx31 is 270 amino acids long and is coded for by a single copy gene. It is expressed as a 1.7-kilobase mRNA that is detected in placenta, Harderian gland, skin, and eye. Cx31 is highly conserved and can be detected in species as distantly related to rat as Xenopus laevis. It exhibits extensive sequence similarity to the previously identified connexins, 58, 50, and 40% amino acid identity to Cx26, Cx32, and Cx43, respectively. When conservation of predicted phosphorylation sites is used to adjust the alignment of Cx31 to other connexins, a unique alignment of three predicted protein kinase C phosphorylation sites near the carboxyl terminus of Cx31 with three sites at the carboxyl terminus of Cx43 is revealed.
Additional Information
© 1991 American Society for Biochemistry and Molecular Biology. Received for publication, September 24, 1991. We thank Dr. D. Paul for the Cx32 cDNA clone, Dr. E. Beyer for the Cx43 cDNA, Dr. B. Nicholson for the Cx26 cDNA, Dr. J. Bonner for the rat genomic library, and R. Wagner for the Xenopus genomic DNA. We are also grateful to Dr. B. Yancey and Dr. K. Puranam for comments and suggestions on the manuscript. This work was supported in part by Grants HL37109 and BRSGRR07003 from the National Institutes of Health and the Ruddock Fund. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. The nucleotide sequence(s) reported in this paper has been submitted to the GenBank(TM)/EMBL Data Bank with accession number(s) M59936.Attached Files
Published - HOHjbc91.pdf
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Additional details
- Eprint ID
- 12104
- Resolver ID
- CaltechAUTHORS:HOHjbc91
- National Institutes of Health
- HL37109
- National Institutes of Health
- BRSGRR07003
- Ruddock Fund, Caltech
- Created
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2008-10-31Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field