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Published 2001 | public
Journal Article

Computationally Focusing the Directed Evolution of Proteins


Directed evolution has proven to be a successful strategy for the modification of enzyme properties. To date, the preferred experimental procedure has been to apply mutations or crossovers randomly throughout the gene. With the emergence of powerful computational methods, it has become possible to develop focused combinatorial searches, guided by computer algorithms. Here, we describe several computational methods that have emerged to aid the optimization of mutant libraries, the targeting of specific residues for mutagenesis, and the design of recombination experiments.

Additional Information

© 2002 Wiley-Liss, Inc. Received 18 September 2001; Accepted 19 September 2001. Article first published online: 29 Jan 2002.

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August 21, 2023
October 24, 2023