Inhibition of Neural Crest Cell Attachment by Integrin Antisense Oligonucleotides
- Creators
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Lallier, Thomas
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Bronner-Fraser, Marianne
Abstract
Neural crest cell interactions with extracellular matrix molecules were analyzed with the use of antisense oligonucleotides to block synthesis of integrin subunits. When added to the culture medium of quail neural crest cells, selected antisense phosphorothiol oligonucleotides reduced the amounts of cell surface ɑ or β integrin subunits by up to 95 percent and inhibited neural crest cell attachment to laminin or fibronectin substrata. Differential effects on specific alpha integrins were noted after treatment with ɑ-specific oligonucleotides. Cells recovered the ability to bind to substrata 8 to 16 hours after treatment with inhibitory oligonucleotides. The operation of at least three distinct ɑ integrin subunits is indicated by substratum-selective inhibition of cell attachment.
Additional Information
© 1991 American Association for the Advancement of Science. 22 April 1992; accepted 2 December 1992. We thank S. Fraser for comments on the manuscript. Supported by USPHS-15527 to MB-F.Additional details
- Eprint ID
- 65970
- Resolver ID
- CaltechAUTHORS:20160406-140352440
- NIH
- HD-15527
- Created
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2016-04-06Created from EPrint's datestamp field
- Updated
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2022-12-23Created from EPrint's last_modified field