Microarray Transcription Profiling of a Shewanella oneidensis etrA Mutant
DNA microarrays were used to examine the effect of an insertional mutation in the Shewanella oneidensis etrA (electron transport regulator) locus on gene expression under anaerobic conditions. The mRNA levels of 69 genes with documented functions in energy and carbon metabolism, regulation, transport, and other cellular processes displayed significant alterations in transcript abundance in an etrA-mutant genetic background. This is the first microarray study indicating a possible involvement of EtrA in the regulation of gene expression in S. oneidensis MR-1.
Additional Information© 2002, American Society for Microbiology. Received 17 January 2002/ Accepted 14 May 2002 We thank Guangshan Li for PCR amplification of S. oneidensis MR-1 ORFs, John Heidelberg for S. oneidensis MR-1 genome sequence information, Craig Brandt for help with statistical analysis of the microarray data, and Allison Murray for advice on microarray data analysis. This research was supported by the Microbial Cell Project, the Microbial Genome Program, the Natural and Accelerated Bioremediation Research Program, Office of Biological and Environmental Research, Office of Science, the U.S. Department of Energy. Oak Ridge National Laboratory is managed by the University of Tennessee—Battelle under contract DE-AC05-00OR22464.
Published - BELjbact02.pdf