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Published 2005 | public
Book Section - Chapter

Functional Analysis of Detergent-Solubilized and Membrane-Reconstituted ATP-Binding Cassette Transporters


ATP-binding cassette (ABC) transporters are vital to any living system and are involved in the translocation of a wide variety of substances, from ions and nutrients to high molecular weight proteins. This chapter describes methods used to purify and membrane reconstitute ABC transporters in a fully functional state. The procedures are largely based on our experience with substrate-binding protein-dependent ABC uptake systems from bacteria, but the approaches should be applicable to multisubunit membrane complexes in general. Also, we present simple methods, based on substrate binding or translocation, to follow the activity of the protein complexes in detergent-solubilized and/or membrane-reconstituted state(s).

Additional Information

Copyright 2005, Elsevier Inc. All rights reserved. This work was supported by "Top-subsidie" Grant 700-50-302 from the Netherlands Organization for Scientific Research-Chemische Wetenschappen, a Fulbright grant from NACEE, and EU-FP6 (E-Mep programme). We thank Nushin Aghajari, Arnold Driessen, Marc LeMaire, and Gea Schuurman-Wolters for sharing information prior to publication.

Additional details

August 19, 2023
January 13, 2024