Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published August 16, 1974 | public
Journal Article

Very long stretches of free DNA in chromatin


The arrangement of histones along the DNA in chromosomes has been studied using digestion of the chromatin with DNase and titration of the chromatin with polylysine or certain dyes as probes. The precision of these probes is limited by the extent to which DNA partially covered with histones will react with the enzymes or titration agents used. Furthermore, it has been noted that histones are redistributed along or between DNA fibres under conditions arising during the normal course of isolation and handling of the chromatin. This phenomenon may have strongly influenced the results obtained previously.

Additional Information

© 1974 Nature Publishing Group. Received May 1, 1973; revised January 31, 1974. We thank V. V. Bakayev and A. A. Bayev, jun. for collaboration at later stages of the work, Dr V. N. Soyfer for providing ultracentrifuge facilities and N. N. Dohbert for technical assistance.

Additional details

August 19, 2023
October 23, 2023