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Published May 1, 2004 | public
Journal Article Open

Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis


We have developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab-on-a-chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications.

Additional Information

© 2004 Oxford University Press Received February 27, 2004; Revised and Accepted April 26, 2004; Published online 20 May 2004 The authors thank Markus Enzelberger and Marc Unger for fruitful discussions of chemistry. The authors also thank Todd Thorsen for technical help with the microfluidic chips fabrication and testing. Partial support for this work was provided by NIH grant HG01642, DARPA grant DAAD19-001-0392, and NIH training grant 5T32-GM07616.


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