A Calcium-Sensitive Magnetic Resonance Imaging Contrast Agent
- Creators
- Li, Wen-hong
- Fraser, Scott E.
- Meade, Thomas J.
Abstract
Ca^(2+) is an important intracellular secondary messenger of signal transduction. Changes in the cytosolic concentration of Ca^(2+) trigger changes in cellular metabolism and are responsible for cell signaling and regulation. Advances in optical microscopy techniques and improvements in fluorescent dyes capable of measuring Ca^(2+) concentration have added greatly to the understanding of the critical role this ion plays in cell and neurobiology. However, a fundamental limitation of light-based microscope imaging techniques employing dyes or fluorochromes is that they produce toxic photobleaching byproducts and are limited by light scattering to those cells within 100 µm of the surface. Magnetic resonance imaging (MRI) of biological structures provides an alternative to light-based microscopy that can circumvent these limitations. Recent work in this area has demonstrated the feasibility of true three-dimensional MR imaging at cellular resolution (~10 µm).
Additional Information
© 1999 American Chemical Society. Publication Date (Web): January 29, 1999. This work was supported by the Biological Imaging Center of the Beckman Institute and the Human Brain Project with contributions from the National Institute on Drug Abuse, the National Institute of Mental Health, and the National Science Foundation.Additional details
- Eprint ID
- 86511
- DOI
- 10.1021/ja983702l
- Resolver ID
- CaltechAUTHORS:20180521-141013911
- Caltech Beckman Institute
- Human Brain Project
- National Institute on Drug Abuse
- National Institute of Mental Health (NIMH)
- NSF
- NIH
- Created
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2018-05-21Created from EPrint's datestamp field
- Updated
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2021-11-15Created from EPrint's last_modified field