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Published May 10, 2019 | Accepted Version + Supplemental Material
Journal Article Open

Particulate methane monooxygenase contains only mononuclear copper centers


Bacteria that oxidize methane to methanol are central to mitigating emissions of methane, a potent greenhouse gas. The nature of the copper active site in the primary metabolic enzyme of these bacteria, particulate methane monooxygenase (pMMO), has been controversial owing to seemingly contradictory biochemical, spectroscopic, and crystallographic results. We present biochemical and electron paramagnetic resonance spectroscopic characterization most consistent with two monocopper sites within pMMO: one in the soluble PmoB subunit at the previously assigned active site (Cu_B) and one ~2 nanometers away in the membrane-bound PmoC subunit (Cu_C). On the basis of these results, we propose that a monocopper site is able to catalyze methane oxidation in pMMO.

Additional Information

© 2019 American Association for the Advancement of Science. This is an article distributed under the terms of the Science Journals Default License. Received 29 August 2018; accepted 15 April 2019. We thank G. E. Cutsail III, M. A. Culpepper, and H. W. Pinkett for helpful discussions as well as an anonymous reviewer for the expert analysis of the Gaussian fitting and providing above and beyond effort. This work was supported by NIH grants GM118035 (A.C.R.), GM111097 (B.M.H.), and 5T32GM008382 (M.O.R.) and NSF grant 1534743 (S.L.M., B.D.O., and A.C.R.). F.M. was supported by a Royal Society Wolfson Research Merit Award. Author contributions: M.O.R, F.M., A.N., T.J.L., B.D.O, S.L.M., A.C.R., and B.M.H. designed experiments. M.O.R., F.M., J.W., and A.N. carried out experiments. M.O.R, A.C.R., and B.M.H. wrote the manuscript. Competing interests: A patent related to this work has been issued: A. C. Rosenzweig, T. J. Lawton, A. Nisthal, J. S. Kostecki, H. K. Privett, F. Lee, B. Olafson, A. D. Dousis, "Engineered recombinant enzymes for methane oxidation" U.S. patent US9896700B2. B.D.O. is a cofounder and the CEO of Protabit, a for-profit company that develops and markets software for protein engineering and computational protein design. S.L.M. is a cofounder of Protabit. Data and materials availability: All data are available in the manuscript or the supplementary materials.

Attached Files

Accepted Version - nihms-1043022.pdf

Supplemental Material - aav2572-Ross-SM.pdf


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August 19, 2023
October 20, 2023