CaltechAUTHORS
Published March 1995 | Version public
Journal Article Metadata-only

Genomic imprinting of Mash2, a mouse gene required for trophoblast development

Creators

  • 1. ROR icon Lunenfeld-Tanenbaum Research Institute
  • 2. ROR icon Institute of Genetics and Molecular and Cellular Biology
  • 3. ROR icon Princeton University
  • 4. ROR icon Frederick National Laboratory for Cancer Research
  • 5. ROR icon California Institute of Technology
  • 6. ROR icon University of Toronto
  • 7. ROR icon New York University Langone Medical Center

Abstract

The mouse gene Mash2 encodes a transcription factor required for development of trophoblast progenitors. Mash2- homozygous mutant embryos die at 10 days post−coitum from placental failure. Here we show that Mash2 is genomically imprinted. First, Mash2+/- embryos inheriting a wild−type allele from their father die at the same stage as -/- embryos, with a similar placental phenotype. Second, the Mash2 paternal allele is initially expressed by groups of trophoblast cells at 6.5 and 7.5 days post−coitum, but appears almost completely repressed by 8.5 days post−coitum. Finally, we have genetically and physically mapped Mash2 to the distal region of chromosome 7, within a cluster of imprinted genes, including insulin−2, insulin−like growth factor−2 and H19.

Additional Information

©.1995 Nature Publishing Group. Received 23 November; accepted 1 January 1995. We thank K. Harpal, A. Freer and B. Cho for excellent technical assistance. This research was supported in part by grants from the National Cancer Institute of Canada and Bristol-Myers Squibb Limited (A.L.J.), the Medical Research Council of Canada (J.R.), an ATIPE from Centre National de la Recherche Scientifique (CNRS)(F.G.), a grant from the National Cancer Institute, DHHS under contract NOI-CO-46000 with ABL (N.G.C.,N.A.J.). F.G. was supported in part by a fellowship from the MRC of Canada and CNRS, S.M.T. is an investigator, D.J.A. is an Associate Investigator and J.R. and A.L.J. are International Scholars of the Howard Hughes Medical Institute. J.R. is a Terry Fox Research Scientist of the NCIC and A.L.J. was a MRC Scientist.

Additional details

Identifiers

Eprint ID
56815
DOI
10.1038/ng0395-235
Resolver ID
CaltechAUTHORS:20150421-114131586

Related works

Describes
10.1038/ng0395-235 (DOI)

Funding

National Cancer Institute of Canada
Bristol-Myers Squibb Limited
Medical Research Council of Canada
Centre National de la Recherche Scientifique (CNRS)
National Cancer Institute
NOI-CO-46000
Howard Hughes Medical Institute (HHMI)

Dates

Created
2015-04-21
Created from EPrint's datestamp field
Updated
2021-11-10
Created from EPrint's last_modified field