Published July 10, 2003
| Published
Book Section - Chapter
Open
New implementation of second harmonic generation microscopy for three-dimensional resolution
- Creators
-
Yang, Changhuei
- Mertz, Jerome
- Others:
- Periasamy, Ammasi
- So, Peter T. C.
Abstract
We present a fast scanning transmission-mode confocal scanning laser microscope system based on the use of a second harmonic generation (SHG) crystal for signal detection. The quadratic intensity dependence of SHG is exploited to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal plays the role of a virtual pinhole that remains self-aligned without a need for de-scanning. We demonstrate that this new microscope method produces images with higher contrast and less speckle than transmission scanning microscopy with linear detection.
Additional Information
© 2003 Society of Photo-Optical Instrumentation Engineers (SPIE). We thank L. Moreaux for initial work on this project. C. Y. is grateful to INSERM (France) and ASTAR (Singapore) for financial support and fellowship award.Attached Files
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Additional details
- Eprint ID
- 88000
- Resolver ID
- CaltechAUTHORS:20180719-111608529
- Institut national de la santé et de la recherche médicale (INSERM)
- Agency for Science, Technology and Research (A*STAR)
- Created
-
2018-07-19Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field
- Series Name
- Proceedings of SPIE
- Series Volume or Issue Number
- 4963