Ultrasonic reporters of calcium for deep tissue imaging of cellular signals

Z.J., A.L., and M.G.S. conceived and designed the study. Z.J., and A.L. designed and planned experiments. Z.J., A.L., R.Z., T.A.T., C.R., M.D., R.C.H, D.M., and Y.Y. conducted the experiments. Z.J. wrote the scripts for ultrasound imaging and data processing. Z.J. and Y.Y. analyzed the data. Z.J. wrote the manuscript, with input from all authors. M.G.S. supervised the research. All authors have given approval to the final version of the manuscript.

All plasmids used in this study are available from M.G.S. under a material agreement with the California Institute of Technology. The key genetic constructs will be deposited with Addgene at the time of manuscript publication. Ultrasound acquisition and processing scripts used to generate key figures and results will be posted to a publicly accessible GitHub repository at the time of manuscript publication. Raw data are available from the corresponding author upon reasonable request.

• Supplementary Materials[supplements/566364_file03.pdf]
• Supplementary Video 1[supplements/566364_file04.mp4]

Z.J., M.G.S., A.L., and T.A.T. are inventors on one patent application related to this work filed by California Institute of Technology (no. 17/937,975, filed 1 October 2022). The authors declare that they have no other competing financial interests.