Electrocatalysis in DNA sensors
Electrocatalysis is often thought of solely in the inorganic realm, most often applied to energy conversion in fuel cells. However, the ever-growing field of bioelectrocatalysis has made great strides in advancing technology for both biofuel cells as well as biological detection platforms. Within the context of bioelectrocatalytic detection systems, DNA-based platforms are especially prevalent. One subset of these platforms, the one we have developed, takes advantage of the inherent charge transport properties of DNA. Electrocatalysis coupled with DNA-mediated charge transport has enabled specific and sensitive detection of lesions, mismatches and DNA-binding proteins. Even greater signal amplification from these platforms is now being achieved through the incorporation of a secondary electrode to the platform both for patterning DNA arrays and for detection. Here, we describe the evolution of this new DNA sensor technology.
© 2014 Elsevier Ltd. Received 22 April 2014; Accepted 2 July 2014. Available online 12 July 2014. We are grateful for the financial support of NIH (GM61077) in the evolution of our DNA sensor technology and for the continual great ideas and hard work of our coworkers in creating the many generations of DNA sensors that we explored, some of which was described here. We are grateful also for the great collegiality of John Bercaw over the years, in always asking probing questions to help us focus on mechanism, and thus help clarify the path ahead.
Accepted Version - nihms613444.pdf