Three-dimensional real-time imaging of cardiac cell motions in living embryos
While quantitative analysis of dynamic biological cell motions in vivo is of great biomedical interest, acquiring 3-D (plus time) information is difficult due to the lack of imaging tools with sufficient spatial and temporal resolution. A novel 3-D high-speed microscopic imaging system is developed to enable 3-D time series data acquisition, based on a defocusing technique (DDPIV). Depth coordinate Z is resolved by the triangular image patterns generated by a mask with three apertures forming an equilateral triangle. Application of this technique to microscale imaging is validated by calibration of targets spread over the image field. 1-µm fluorescent tracer particles are injected into the blood stream of 32 h post-fertilization developing zebrafish embryos to help describe cardiac cell motions. 3-D and velocity fields of cardiovascular blood flow and trajectories of heart-wall motions are obtained.
© 2008 Society of Photo-Optical Instrumentation Engineers. Paper 06305RR received Oct. 29, 2006; revised manuscript received Aug. 31, 2007; accepted for publication Sep. 6, 2007; published online Jan. 30, 2008; corrected Jun. 13, 2008. This work was supported by NIH Grant R01RR023190.
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Published - LUJjbo08.pdf
Erratum - LUJjbo08corr.pdf
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