Multiplexed DNA-Modified Electrodes
We report the use of silicon chips with 16 DNA-modified electrodes (DME chips) utilizing DNA-mediated charge transport for multiplexed detection of DNA and DNA-binding protein targets. Four DNA sequences were simultaneously distinguished on a single DME chip with 4-fold redundancy, including one incorporating a single base mismatch. These chips also enabled investigation of the sequence-specific activity of the restriction enzyme Alu1. DME chips supported dense DNA monolayer formation with high reproducibility, as confirmed by statistical comparison to commercially available rod electrodes. The working electrode areas on the chips were reduced to 10 μm in diameter, revealing microelectrode behavior that is beneficial for high sensitivity and rapid kinetic analysis. These results illustrate how DME chips facilitate sensitive and selective detection of DNA and DNA-binding protein targets in a robust and internally standardized multiplexed format.
© 2010 American Chemical Society. Received November 23, 2009. Publication Date (Web): February 4, 2010. This research was supported by the NIH (GM61077). J.D.S. also thanks the National Institute of Biomedical Imaging and Bioengineering for a postdoctoral fellowship. The authors thank J. Genereux, M. Buzzeo, and D. Ceres for fruitful discussions, J. DeFranco for assistance with photolithography. and S. Olson and M. Roy for construction of the test mount and clamp assembly.
Accepted Version - nihms177133.pdf
Supplemental Material - ja909915m_si_001.pdf