Published June 1989
| Version public
Journal Article
Expression of mRNA Encoding Voltage-Dependent Ca Channels in Xenopus Oocytes: Review and Progress Report
Abstract
We are studying electrically excitable Ca channels with an approach that combines nucleic acid molecular biology and membrane biophysics. Gurdon et al. first employed Xenopus oocytes as a system for heterologous expression of RNA. This system was later adapted for electrophysiological studies of ion channels by Barnard, Miledi, and their colleagues. Although other expression systems are being developed for membrane channels [Claudio et al. and Beam et al., this volume], the oocyte is now the most common, robust, and best-characterized system. Experiments in which channels are expressed by RNA injections in oocytes have at least three major goals.
Additional Information
© 1989 New York Academy of Sciences. This research was sponsored by grants from the National Institutes of Health (GM‐10991 and GM‐29836), from the United States—Israel Binational Science Foundation, from the CNRS (LP 8402), and from INSERM (U249), by postdoctoral fellowships from the American Heart Association to T. S. and J. P. L., from the NIH to B. M. C., and by a Bantrell Fellowship to N. D. J. N. thanks Laboratoire Servier for a travel grant.Additional details
Identifiers
- Eprint ID
- 106003
- DOI
- 10.1111/j.1749-6632.1989.tb24094.x
- Resolver ID
- CaltechAUTHORS:20201012-160322461
Related works
- Describes
- 10.1111/j.1749-6632.1989.tb24094.x (DOI)
Funding
- NIH
- GM-10991
- NIH
- GM-29836
- Binational Science Foundation (USA-Israel)
- Centre National de la Recherche Scientifique (CNRS)
- LP 8402
- Institut national de la santé et de la recherche médicale (INSERM)
- U249
- American Heart Association
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Laboratoire Servier
Dates
- Created
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2020-10-13Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field