Cleave and Rescue gamete killers create conditions for gene drive in plants

We thank E. Meyerowitz and members of the Meyerowitz Lab Paul Tarr and Carla de Agostini Verna for introducing us to techniques for Arabidopsis maintenance, transgenesis and crossing. This work was supported by a grant to B.A.H. from the Caltech Center for Evolutionary Science (G.O. and M.L.J.) and the Caltech Resnick Sustainability Institute Explorer Grant (G.O.). T.I. was supported by NIH Training grant number 5T32GM007616-39 and with support to B.A.H. from the US Department of Agriculture, National Institute of Food and Agriculture (NIFA) specialty crop initiative under US Department of Agriculture NIFA award number 2012-51181-20086.

All data are available in the main text and the Supplementary Information files. Illumina sequencing reads were deposited to SRA (bioproject, PRJNA1074841). The Arabidopsis TAIR 10 genome assembly was used in this study. Constructs and seeds of transgenic plants created in this study are available upon request.

Extended Data Fig. 1 Alignment of the recoded A. lyrata rescue coding region to the A. thaliana target.

Modelling code and more information on the model, the scripts and parameters used to generate the data, and the data itself can be found at https://github.com/HayLab/Pigss. Plots were generated in R (version 4.2.3) with the ggplot2 package¹²².

The authors have filed patent applications on ClvR and related technologies (US application numbers 15/970,728 and 16/673,823).