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Published July 1, 1959 | public
Journal Article Open

The relative homogeneity of microbial DNA


Density-gradient centrifugation has revealed that the population of DNA molecules from E. coli is relatively homogeneous with respect to buoyant density in a solution of cesium chloride.(1) Because of the notably small apparent atomic volume of nitrogen in aqueous solutions of organic compounds(2) and because the guanine-cytosine base pair is more rich in nitrogen than is the adenine-thymine pair, it was considered that the density homogeneity among E. coli DNA molecules might reflect a high degree of homogeneity with respect to base composition. To investigate this possibility, an examination has been made of the relationship between buoyant density in cesium chloride solution and base composition of DNA from various sources.

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© 1959 by the National Academy of Sciences. Communicated by Linus Pauling, May 6, 1959. Aided by a grant from the National Institutes of Health. [R.R. was a] Postdoctoral Fellow fo the U.S. Public Health Service. Gates and Crellin Laboratories of Chemistry, Contribution No. 2460.


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