Polyphosphate affects cytoplasmic and chromosomal dynamics in nitrogen-starved Pseudomonas aeruginosa
Abstract
Copyright and License
© 2024 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
Acknowledgement
We thank the Center for Environmental Microbial Interactions (CEMI) at Caltech, where the project was conceived. We are grateful to all members of the Manley and Racki groups for insightful discussions. We thank Amanda Habel for help with optimizing the luciferase assay and optimizing both acquisition and tracking parameters for the chromosome and μNS particles. We thank Ravi Chawla for testing our tracking parameters on carbon starvation data. We thank Megan Bergkessel for helpful feedback on the manuscript. Funding was received from the NIH (DP2-GM-140918 to L.R.R.), European Research Council (ERC CoG 819823, Piko to S. Manley and S. Magkiriadou), the Swiss NSF (182429 to S. Manley and W.L.S.), and the Donald E. and Delia B. Baxter Foundation Fellowship (L.R.R.). This is manuscript #30145 from The Scripps Research Institute.
Contributions
D.K.N., S. Manley, and L.R.R. conceived of research and administered the project; S. Magkiriadou, W.L.S., D.K.N., S. Manley, and L.R.R. designed research; L.R.R. performed research and contributed new reagents; S. Magkiriadou, and W.L.S. developed software; S. Magkiriadou, W.L.S., and L.R.R. analyzed data; S. Magkiriadou, W.L.S., S. Manley, and L.R.R. validated analysis; S. Magkiriadou, W.L.S., D.K.N., S. Manley, and L.R.R. reviewed and edited the paper; S. Magkiriadou, S. Manley, and L.R.R. wrote the paper.
Data Availability
Values used in graphs; code data have been deposited in Zenodo; Github (https://doi.org/10.5281/zenodo.5772215 (45) and https://github.com/LEB-EPFL/tracking-and-diffusion (46)).
Conflict of Interest
The authors declare no competing interest.
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Additional details
- ISSN
- 1091-6490
- PMCID
- PMC11009631
- California Institute of Technology
- Caltech Center for Environmental and Microbial Interactions
- National Institutes of Health
- DP2-GM-140918
- European Research Council
- 819823
- Swiss National Science Foundation
- 182429
- Donald E. and Delia B. Baxter Foundation
- Caltech groups
- Division of Biology and Biological Engineering, Caltech Center for Environmental Microbial Interactions (CEMI), Division of Geological and Planetary Sciences