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Published October 2021 | Accepted Version + Supplemental Material
Journal Article Open

Chloroplast SRP43 autonomously protects chlorophyll biosynthesis proteins against heat shock

Abstract

The assembly of light-harvesting chlorophyll-binding proteins (LHCPs) is coordinated with chlorophyll biosynthesis during chloroplast development. The ATP-independent chaperone known as chloroplast signal recognition particle 43 (cpSRP43) mediates post-translational LHCP targeting to the thylakoid membrane and also participates in tetrapyrrole biosynthesis (TBS). How these distinct actions of cpSRP43 are controlled has remained unclear. Here, we demonstrate that cpSRP43 effectively protects several TBS proteins from heat-induced aggregation and enhances their stability during leaf greening and heat shock. While the substrate-binding domain of cpSRP43 is sufficient for chaperoning LHCPs, the stabilization of TBS clients requires the chromodomain 2 of the protein. Strikingly, cpSRP54—which activates cpSRP43's LHCP-targeted function—inhibits the chaperone activity of cpSRP43 towards TBS proteins. High temperature weakens the interaction of cpSRP54 with cpSRP43, thus freeing cpSRP43 to interact with and protect the integrity of TBS proteins. Our data indicate that the temperature sensitivity of the cpSRP43–cpSRP54 complex enables cpSRP43 to serve as an autonomous chaperone for the thermoprotection of TBS proteins.

Additional Information

© 2021 Nature Publishing Group. Received 03 February 2021; Accepted 28 July 2021; Published 02 September 2021. We thank D. Schünemann (Ruhr-Universität Bochum) for providing us the chaos and ffc mutants, the GST–cpSRP43 expression vector, and antibodies against cpSRP43 and cpSRP54, and P. Hardy for critical reading of the manuscript. This work was supported by a grant from the Chinese Scholarship Council to S.J., grant nos R35 GM136321 and DOE.DE-SC0020661 to A.S. and S.S., and grants from the Deutsche Forschungsgemeinschaft to B.G. (nos FOR2092, GR 936/18-1 and SFB TRR175, subproject C04) and to P.W. (no. WA 4599/2-1). Data availability: The data supporting the findings of this study are available within the article and its Supplementary Information. Source data are provided with this paper. These authors contributed equally: Shuiling Ji, Alex Siegel. Author Contributions: B.G. and P.W. designed the experiments. S.J. and A.S. performed the experiments. S.J., A.S., S.S., B.G. and P.W. analysed the data. B.G. and P.W. wrote the manuscript. S.J., A.S. and S.S. gave critical comments and revisions to the manuscript. The authors declare no competing interests. Peer review information: Nature Plants thanks Briardo Llorente, Tatsuru Masuda and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

Attached Files

Accepted Version - nihms-1780143.pdf

Supplemental Material - 41477_2021_994_Fig10_ESM.webp

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Additional details

Created:
August 22, 2023
Modified:
October 23, 2023